• Flippy/Fliqpy - Flippy is a male green-colored bear with a pink heart-shaped nose. He wears a camo jacket, dog tags around his neck, and a green beret with a checkered crest on his head. He has a severe case of post traumatic stress disorder. Whenever he sees or hears something that reminds him of war (e.g., gunshot-like sounds, crackling campfires and ketchup), he goes insane and kills everyone around him (and sometimes himself), believing that he is still in war. While insane, several physical features about him change: his eyes turn a different color, usually green or yellow, his voice changes from high and cute to low and evil, and his teeth become sharp. If he is in his evil state, he has round pupils instead of the standard Pac-Man shaped ones. When he is "flipped-out", he is known as Evil Flippy, or "Fliqpy."
• Flaky - Flaky is a red porcupine (may confuse viewers that she is a hedgehog) whose quills are full of white flakes-like dandruff; thus the name.
• Lumpy - Male - Lumpy is a light blue moose with very low intelligence, bad teeth (although they are clear white), distorted eyes and mismatched antlers (one points up and one points down).
• The Mole - Male - The Mole is a blind dark pink mole with black sunglasses (or possibly teashades) with a mole by his nose. He wears a long purple turtleneck on his sweater. Like Mime and Cro-Marmot, he doesn't speak.
• Lammy & Mr. Pickles - Female / Male - Lammy is a purple lamb with wooly white hair, tail fur and wears a wooly white sweater. Her best friend or her worst enemy is a green pickle who wears a top hat and has a moustache named Mr. Pickels who, is very violent.
• Truffles - Male - Truffles is a bluish-gray pig. (Doesn't Appear In Any Of The Episodes)
• Splendid - Male - Splendid is a pale blue superhero flying squirrel based on DC Comics' Superman with light blue gliding wings, a light blue/white oval marking on his torso, and a red mask around his head.
• Cuddles - Male - Cuddles is a canary-yellow rabbit with pink cheeks and pink bunny slippers. His white, fluffy, curly hair tuft and cotton tail look exactly the same.
• Handy - Male - Handy is an orange beaver with inexplicably amputated hands. The stumps on the ends of his arms are wrapped with bandages. He wears a tool belt and a yellow worker's helmet.
• Russell - Male - Russell is a turquoise sea otter with typical pirate accessories like a Jolly Roger's hat, a striped red/white shirt, a hook, an eyepatch and two wooden peglegs.
• Toothy - Male - Toothy is a light purple/mauve beaver with very large, gapped teeth (from which his name is derived), which appears to be diastema and freckles.
• Giggles - Female - Giggles is a pink chipmunk who has a white diamond-shaped marking, a white oval on her torso and wears a big red bow on her head.
• Buddhist Monkey - Male - Buddhist Monkey is a yellow Buddhist monk monkey.
• Cro-Marmot - Male - Cro-Marmot is a caveman-like, greenish-yellow marmot frozen in a block of ice who wears a leopard skin loincloth and holds a wooden club in his hand.
• Pops & Cub - Male / Male - Pop & Cub are two tan father-and-son bears, Pop appears wearing a 50's kind, also he is always seen smoking a pipe while Cub wears a diaper and a beanie.
• Lifty & Shifty - Male / Male - Lifty & Shifty are two green twin gangster raccoon twin brothers who frequently steal merchandise from the other Happy Tree Friends (thus their names), Shifty wears a fedora.
• Mime - Male - Mime is a purple male roe deer with pointed ears, and antlers who wears face makeup and a navy blue/dark purple and white, striped shirt. He never speaks.
• Nutty - Male - Nutty is a light green crazy squirrel with one yellow diamond-shaped marking on his head and a large, curled-up tail that wags when he's excited. Nutty has an addiction for sugar. He has a big swirly lollipop, a candy cane, small Dum Dum Pop, and a candy apple stuck to his fur, which he even treats as his wardrobe.
• Disco Bear - Male - Disco Bear is a light orange-colored bear with an orange afro and sideburns who likes to dance to disco music (thus his name). He wears 1970s style clothing, including a yellow leisure jacket, yellow bell-bottom pants, and orange and white dancing platform shoes.
• Petunia - Female - Petunia is an indigo and dark blue skunk with a sky blue arrow marking on her forehead, which is connected to the triangle-shaped marking on her back and sky blue lines on her long tail. She always wears a pink flower on top of her head and a pine scent car air freshener or deodorizer around her neck.
• Sniffles - Male - Sniffles is a scientist blue-gray anteater who is the more intelligent character in the series.
• The Ant Family - There is a mother ant, a baby ant, a son, and a daughter. They live an anthill with a door at the top.